Investigation of novel extremely halotolerant bacteria and polyglucan hydrolytic enzymes
Date Issued
2009
Date
2009
Author(s)
Wang, Chung-Yi
Abstract
In the coastal and marine areas of southern Taiwan, various solar salt fields have been established. Since ancient time, Tainan has already known as key salt production site. Discovery of these microbial information in these unique ecosystems is rather encouraging for further novel species discovery, exploration and conservation of useful microbes. In the first section, the microbiota diversity of the former salterns was investigated by denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH). Soil samples from three salterns were analyzed using DGGE representing 5 archaea and 18 bacteria taxonomic groups, whereas 8.7% of microbes were unclassified microorganisms. During FISH analysis, several taxonomic-specific probes were used. Archaea occupied 2.7–6.6% whereas bacteria accounted for 37.2–52.9% of total microbial population at the three sites. Among these sampling sites, the Szutsao saltern had the highest diversity in halophilic microbial composition, as indicated by DGGE and FISH. In the second section, we describe two novel species that were isolated from the Chigu and Szutsao saltern. On the basis of phenotypic, phylogenetic and genetic analyses, these two strains were considered to represent a novel species of the genus Virgibacillus and Marinobacter, and were named Virgibacillus chiguensis and Marinobacter szutsaonensis according to Chigu and Szutsao saltern. These two strains were deposited in culture collection center. In the last section, we reported the characteristics of the two polyglucan hydrolytic enzymes (cellulase and xylanase) were purified from the halophilic bacterium Salinivibrio sp. NTU-05 and Bacillus sp. NTU-06. These enzymes were active over a range of salt concentration (0–25% NaCl) when examined in a culture broth. Some of the novel characteristics of these halostable enzymes indicate that they may have potential applications in treating agricultural waste and in the bioremediation of cellulose materials.
Subjects
Microbial diversity
extremely halotolerant bacteria
polyglucan hydrolytic enzymes
SDGs
Type
thesis
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