Fermented Black Soybean Inhibits LDL Oxidation, Colon Cancer Cell Proliferation and Genotoxicity of 4-Nitroquinoline-N-oxide on Human Intestine Cell
Date Issued
2010
Date
2010
Author(s)
Chen, Yu-Fei
Abstract
In the present study, various solvent (water, 80% methanol, 80% ethanol) extracts of Aspergillus awamori-fermented black soybean were examined for total phenolic, amino nitrogen contents and the antioxidant activities including Cu2+-chelating ability and inhibition on low density lipoprotein (LDL) oxidation. In addition, the effect of fermented black soybean extracts against the proliferation of human colon cancer cells Caco-2 and HT-29 as well as on the cytotoxicity and genotoxicity induced by 4-nitroquinoline-N-oxide (4NQO) toward Int-407 were detected. Furthermore, possible anticytotoxicic mechanisms of methanol extract of fermented black soybean were investigated by section-preincubation test.
The results showed that the extracts of the black soybeans, regardless of fermentation, all exhibited antioxidant activities and inhibitory effect on the proliferation of cancer cells as well as cell damage induced by 4NQO. Among the various extracts examined, methanol extract showed the highest contents of total phenolics, while water extract possessed the highest amino nitrogen contents after fermentation. It was found that the water extract of A. awamori-fermented black soybean prepared at 30℃ for 3 days exhibited the highest inhibitory effect on LDL oxidation.
Of the fermented black soybean extracts examed, methanol extract was found to exhibit the highest efficiency in reducing the proliferation of cancer cells. Methanol extract of the fermented black soybean prepared at 30℃ for 3 days was noted to show the highest inhibitory effect on 4NQO-induced cytotoxicity. The extract of fermented black soybean also exhibited a higher protective effect on Int-407 against 4NQO-induced DNA damage than did the respective extract of the non-fermented black soybean. Further study on the possible anticytotoxicic mechanism, revealed that the destoxic, blocking and bio-antitoxic effect all contributed to the anticytotoxicity of A. awamori-fermented black soybean against 4-NQO.
Subjects
black soybean
extraction solvent
low density lipoprotein
SDGs
Type
thesis
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