A Novel Immunogenic Spore Coat-Associated Protein in Bacillus Anthracis: Characterization Via Proteomics Approaches and a Vector-Based Vaccine System
Resource
PROTEIN EXPRESSION AND PURIFICATION v.57 n.1 pp.72-80
Journal
Protein Expression and Purification
Pages
72-80
Date Issued
2008
Date
2008
Author(s)
LIU, YU-TSUENG
LIN, SHWU-BIN
HUANG, CHENG-PO
Abstract
New generation anthrax vaccines have been actively explored with the aim of enhancing efficacies and decreasing undesirable side effects that could be caused by licensed vaccines. Targeting novel antigens and/or eliminating the requirements for multiple needle injections and adjuvants are major objectives in the development of new anthrax vaccines. Using proteomics approaches, we identified a spore coat-associated protein (SCAP ) in Bacillus anthracis. An Escherichia coli vector-based vaccine system was used to determine the immunogenicity of SCAP. Mice generated detectable SCAP antibodies three weeks after intranasal immunization with an intact particle of ultraviolet (UV)- irradiated E coli vector overproducing SCAP. The production of SCAT antibodies was detected via western blotting and SCAP-spotted antigen-arrays. The adjuvant effect of a UV- irradiated E. coli vector eliminates the necessity of boosting and the use of other immunomodulators which will foster the screening and manufacturing of new generation anthrax vaccines. More importantly, the immunogenic SCAP may potentially be a new candidate for the development of anthrax vaccines.
Subjects
immunogenic
spore coat
Bacillus anthracis proteomics
vector
vaccine
SDGs
Other Subjects
anthrax vaccine; bacterial protein; bacterium antibody; recombinant protein; amino acid sequence; animal; anthrax; article; Bacillus anthracis; bacterial spore; blood; chemistry; Escherichia coli; gene vector; genetics; immunology; intranasal drug administration; isolation and purification; metabolism; methodology; molecular genetics; mouse; mouse strain; proteomics; radiation exposure; sequence homology; ultraviolet radiation; Administration, Intranasal; Amino Acid Sequence; Animals; Anthrax; Anthrax Vaccines; Antibodies, Bacterial; Bacillus anthracis; Bacterial Proteins; Escherichia coli; Genetic Vectors; Mice; Mice, Inbred Strains; Molecular Sequence Data; Proteomics; Recombinant Proteins; Sequence Homology, Amino Acid; Spores, Bacterial; Ultraviolet Rays; Bacillus anthracis; Bacteria (microorganisms); Escherichia coli; Mus