Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
Journal
British Journal of Cancer
Journal Volume
80
Journal Issue
3-4
Pages
468-476
Date Issued
1999
Author(s)
Lin Y.-W.
Chou H.-C.
Lin Y.-H.
Lin J.-T.
Lu F.-J.
Abstract
Elucidation of the basic genetic changes of human hepatocellular carcinoma is important for the understanding and treatment of this cancer. We used microsatellite polymorphism markers to study 30 cases of hepatocellular carcinoma (34 tumours) on all human chromosomes. DNA from 34 pairs of hepatocellular carcinomas and corresponding non-tumour parts was prepared. Loss of heterozygosity (LOH) and microsatellite instability on 23 chromosomes were investigated by 231 sets of microsatellite markers. More than 20% LOH was shown for loci on 16q (47.1%), 13q (32.4%), 17p (32.4%), 5q (26.5%), 11p (23.5%) and 9p (20.6%). The commonly affected regions were mapped to 16q12.1, 16q12.2, 16q24, 13q12.1-32, 17p13, 5q32, 5q34, 5q3, 11p15, 33q23-24 and 9p21, Hepatitis B virus carriers had a significantly higher frequency of LOH on chromosomes 5q, 11p and 16q. Furthermore, larger tumour size tended to have higher frequency of LOH at D16S409 locus (16q12.1). Microsatellte instability was only found in 12 of 231 markers and the frequency is very low. These data suggest that the chromosomes 16q, 13q, 17p, 5q, 11p and 9p might participate in hepatocarcinogenesis. However, microsatellite instability might play little role in the development of this cancer in Taiwan.
SDGs
Other Subjects
DNA; adult; aged; article; chromosome 11p; chromosome 13q; chromosome 16q; chromosome 17p; chromosome 5q; chromosome 9p; chromosome map; female; genetic marker; genetic polymorphism; hepatitis b virus; heterozygosity loss; human; liver cell carcinoma; male; microsatellite instability; priority journal; Taiwan; virus carrier; Adult; Aged; Carcinoma, Hepatocellular; Chromosomes, Human; Female; Gene Deletion; Genes, Tumor Suppressor; Humans; Liver Neoplasms; Loss of Heterozygosity; Male; Microsatellite Repeats; Middle Aged; Polymorphism, Genetic; X Chromosome
Type
journal article