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The Immunomodulatory Effects of Areca Nut Extracts and Arecoline on Human Peripheral Monocyte-derived Dendritic Cells
Date Issued
2004
Date
2004
Author(s)
Chen, Yung-Li
DOI
zh-TW
Abstract
The effect of areca nut extracts (ANE) and arecoline (AR) on the differentiation and activation of dendritic cells (DC) was investigated in the present studies. Monocytes isolated from human peripheral blood were cultured in RPMI medium supplemented with GM-CSF and IL-4 to generate DC. Several lines of evidence suggested that the differentiation of DC from monocytes was suppressed by the presence of ANE or AR. First, the cell number of immature DC obtained following 7-day culture of monocytes was decreased in the presence of ANE (10 - 20 mg/mL) or AR (10 - 100 mM). Second, the viability of DC was decreased by ANE treatment. Lastly, the expression of surface markers of DC was influenced by ANE or AR treatment as determined by flow cytometry. The expression of CD40 on total harvested cells was decreased by ANE (20 mg/mL), and the expression of HLA-DR and CD1a was attenuated by AR (100 mM). Similar results were also observed in selected cell populations with larger size. The morphological studies revealed that a higher percentage of iDC remained in round shape in the presence of ANE (25 mg/mL) or AR (500 mM), as compared to the control group. However, the metabolic activity of iDC was not significantly affected by ANE treatment as measure by a MTT assay. In contrast, the metabolic activity of iDC was suppressed by treatments with AR (50 - 1000 mM). Furthermore, the effect of ANE and AR on the reactivity of iDC was studied. Both ANE and AR did not affect the expression of cell surface markers on iDC stimulated with LPS. However, the induction of IL-12B mRNA expression by LPS in iDC was markedly suppressed by pretreatment of cells with ANE (20 mg/mL) or AR (10 - 100 mM). Taken together, the present studies demonstrated that the differentiation of iDC from monocytes and the activation of iDC were significantly suppressed by the presence of ANE and AR.
Subjects
檳榔萃取物
樹狀細胞
成熟
檳榔鹼
脂多醣
免疫調控
分化
介白素-12
areca nut extract
interleukin-12
dendritic cell
differentiation
lipopolysaccride
maturation
arecoline
immunomodulation
Type
thesis
File(s)
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Name
ntu-93-R87629019-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):019291ebb25cd5096b87ba7e88b6fecd