Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. School of Veterinary Medicine / 獸醫專業學院
  4. Veterinary Medicine / 獸醫學系
  5. The Immunomodulatory Effects of Areca Nut Extracts and Arecoline on Human Peripheral Monocyte-derived Dendritic Cells
 
  • Details

The Immunomodulatory Effects of Areca Nut Extracts and Arecoline on Human Peripheral Monocyte-derived Dendritic Cells

Date Issued
2004
Date
2004
Author(s)
Chen, Yung-Li
DOI
zh-TW
URI
http://ntur.lib.ntu.edu.tw//handle/246246/60019
Abstract
The effect of areca nut extracts (ANE) and arecoline (AR) on the differentiation and activation of dendritic cells (DC) was investigated in the present studies. Monocytes isolated from human peripheral blood were cultured in RPMI medium supplemented with GM-CSF and IL-4 to generate DC. Several lines of evidence suggested that the differentiation of DC from monocytes was suppressed by the presence of ANE or AR. First, the cell number of immature DC obtained following 7-day culture of monocytes was decreased in the presence of ANE (10 - 20 mg/mL) or AR (10 - 100 mM). Second, the viability of DC was decreased by ANE treatment. Lastly, the expression of surface markers of DC was influenced by ANE or AR treatment as determined by flow cytometry. The expression of CD40 on total harvested cells was decreased by ANE (20 mg/mL), and the expression of HLA-DR and CD1a was attenuated by AR (100 mM). Similar results were also observed in selected cell populations with larger size. The morphological studies revealed that a higher percentage of iDC remained in round shape in the presence of ANE (25 mg/mL) or AR (500 mM), as compared to the control group. However, the metabolic activity of iDC was not significantly affected by ANE treatment as measure by a MTT assay. In contrast, the metabolic activity of iDC was suppressed by treatments with AR (50 - 1000 mM). Furthermore, the effect of ANE and AR on the reactivity of iDC was studied. Both ANE and AR did not affect the expression of cell surface markers on iDC stimulated with LPS. However, the induction of IL-12B mRNA expression by LPS in iDC was markedly suppressed by pretreatment of cells with ANE (20 mg/mL) or AR (10 - 100 mM). Taken together, the present studies demonstrated that the differentiation of iDC from monocytes and the activation of iDC were significantly suppressed by the presence of ANE and AR.
Subjects
檳榔萃取物
樹狀細胞
成熟
檳榔鹼
脂多醣
免疫調控
分化
介白素-12
areca nut extract
interleukin-12
dendritic cell
differentiation
lipopolysaccride
maturation
arecoline
immunomodulation
Type
thesis
File(s)
Loading...
Thumbnail Image
Name

ntu-93-R87629019-1.pdf

Size

23.31 KB

Format

Adobe PDF

Checksum

(MD5):019291ebb25cd5096b87ba7e88b6fecd

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science