EDD及其交互作用蛋白於基因調控中角色的研究與探討
Functional roles of EDD and its associated proteins in regulating gene expression
Date Issued
2006
Date
2006
Author(s)
Lin, Chih-Li
DOI
en-US
Abstract
EDD, a HECT-domain E3 ubiquitin ligase, was identified to be associated with the transcription elongator – FACT complex. Although mammalian EDD has been implicated in cell proliferation, transcriptional regulation, and DNA damage response, its exact cellular function is largely unknown. Our results demonstrated a predominately nuclear matrix localization of EDD and its in vivo association with FACT, thus suggesting EDD’s involvement in chromatin structure regulation. Further characterization of EDD revealed that EDD forms a stable complex with a WD40 domain- and HMG domain-containing protein AND-1. Experiment evidence indicates that AND-1 is a chromatin and nuclear matrix-associated factor. Interestingly, AND-1 possessed a distinct punctate pattern of subnuclear localization, consistent with its interaction with pre-mRNA splicing complexes, nucleolar proteins, and nuclear lamins. These observations collectively imply that that AND-1 may be a multifunctional protein that links and coordinates transcriptional and post-transcriptional events. Our present study uncovered two key proteins that may be important regulators of gene expression, and putatively linked ubiquitination to such cellular process. Future work on the functional characterization of EDD, AND-1, and their interaction is discussed.
Subjects
轉錄作用
染色質
組蛋白
修剪作用
泛素
泛素化接合?
transcription
chromatin
histone
splicing
ubiquitin
E3 ligase
Type
other
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