Characterization and application of monoclonal antibodies against white spot syndrome virus
Resource
Journal of Fish Diseases 24 (3): 143-150
Journal
Journal of Fish Diseases
Journal Volume
24
Journal Issue
3
Pages
143-150
Date Issued
2001
Date
2001
Author(s)
Abstract
Three hybridoma clones secreting monoclonal antibodies (mabs) were produced from mouse myeloma and spleen cells immunized with white spot syndrome virus (wssv) isolated and purified from Penaeus monodon (fabricius), collected from north-eastern Taiwan. By sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page), the protein profile of this isolate contained four major proteins with sizes of approximately 35 (vp35), 28 (vp28), 24 (vp24), and 19 kda (vp19). Western blot analysis revealed that two Mabs (1d7 and 6e1) recognized epitopes on Vp28 and one Mab (3e8) recognized an epitope on Vp19. The Mab 6e1 isotyped to the Igg1 class was used in both an indirect immunofluorescence assay (IFA) and in an immunochemical staining protocol for successful identification and localization of WSSV in infected shrimp tissues. Antigenic similarity of isolates from Indonesia and Malaysia to the Taiwan isolate was illustrated by IFA with MAb 6E1. A MAb (2F6) which bound specifically to two shrimp proteins, 75 and 72 kDa, and reacted to the healthy and non-target tissues of WSSV in infected shrimp, such as hepatopancreas, is also described here and shows the necessity for specific identification of antibodies.
Subjects
Diagnosis; IFA; MAbs; Penaeus monodon; White spot syndrome virus; WSSV
SDGs
Other Subjects
antigenicity; cell adhesion; epitope; hybridoma; immunofluorescence; Indonesia; Malaysia; molecular size; monoclonal antibody; myeloma cell; SDS polyacrylamide gel electrophoresis; spleen cell; Taiwan; virus characterization; western blotting; white spot syndrome virus; Penaeus monodon
Type
journal article
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