Application of Carbon Nanotube/Chitosan composites by the electrical stimulation in the Proliferation and Differentiation of PC12 Cells
Date Issued
2011
Date
2011
Author(s)
Chen, Tuan-Hao
Abstract
Nerve conduit is a method with potential to replace autologous nerve grafts, serving as the bridge to connect interface of two fracture nerves, and conducting the repairing procedure of the fracture nerves. It is also verified by many researches that by the low-frequency electrical stimulation, the cell membrane potential and permeability will change and possibility of nerve impulse will increase and further facilitate the nerve cell regenerating. If combining the assumptions stated above, it is expected to get better result of the nerve repairing.
In this research, coagulation method is used to make the multi-walled carbon nanotube(MWCNT)/ chitosan compound fiber with the diameter in around 200μm and because the chitosan fiber equips with several advantages including conductivity, biocompatibility, Biodegradable and so on, it is suitable to be applied in the research of how the electricity stimulation facilitate the nerve regeneration.Through the evaluation of different kinds of materials, 0.5wt% MWCNT/ 2wt% chitosan compound is selected as the materials for the in vitro experiment, and the materials and the PC12 cell are co-cultured to evaluate the result of the nerve regeneration. In the experiment, the control parameters are as the following: 20Hz frequency, 50% duty time, one hour stimulation time per day by implementing of the direct current(DC) / alternating current(AC) electricity separately to observe the effect of electricity stimulation to the cell generation.
The result of evaluating the biocompatibility in vitro manifests that multi-walled carbon nanotube/chitosan can provide the cells a great environment to grow up and it is verified that the median neurite length and the direction of growing up have obvious difference after the electricity stimulation experiment, and achieve the great result of the nerve regeneration.
Subjects
Carbon nanotubes
Chitosan
Electrical stimulation
Type
thesis
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