Effects of fresh milk plus Bifido® intake on the intestinal microflora and cytotoxicity on Caco-2 and HT-29
Date Issued
2007
Date
2007
Author(s)
Chyou, Pei-Jiuan
DOI
zh-TW
Abstract
Abstract
The human gastrointestinal is a complex ecosystem, the balance of beneficial bacteria bifidobacteria and harmful Clostridium perfringens is highly related to human health. This study was conducted to investigate the effect of intake fresh milk plus Bifido® on the intestinal microflora, fecal water content, pH values and fecal extracts cytotoxicity on Caco-2 and HT-29 in 24 healthy adults. Furthermore, the changes of viable bifidobacteria, pH value and titratable acidity in fresh milk plus Bifido® store at 4℃ and 11℃ for 20 days were also studied.
Results indicated that intake the fresh milk plus Bifido® significantly increased the colony counts of fecal bifidobacteria (p < 0.05), decreased the growth of C. perfringens (p < 0.05) and significantly increased the log number ratio of bifidobacteria to C. perfringens (p < 0.05). Subjects’ height, weight, BMI, blood parameters, fecal water content and fecal pH had no apparent changes during experimental period. Each period of subjects’ fecal extracts at low concentrations were no cytotoxicity on Caco-2 and HT-29. The cytotoxicity of subjects’ fecal extracts at high concentrations on Caco-2 and HT-29 were significantly reduced during fresh milk plus Bifido® intake periods (p < 0.05). These results suggested that intake the fresh milk plus Bifido® were beneficial in improving the intestinal flora and decreasing cytotoxic materials in the intestinal tract of health adults.
Fresh milk plus Bifido® contains 6.63 log CFU/mL bifidobacteria. The survival number of bifidobacteria in fresh milk plus Bifido® was maintained at 6.2 log CFU/mL during 10 days of shelf life time. The changes of pH value and titratable acidity in fresh milk plus Bifido® did not change statistically during the experimental period (p > 0.05).
Subjects
腸道菌相
雙叉桿菌
產氣莢膜梭菌
細胞毒性
存活率
Intestinal flora
bifidobacteria
Clostridium perfringens
Cytotoxicity
Viability
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94641005-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):f272c16273078532a387ed6ef5c548ea