利用高效液相層析法搭配內標準品定量芝麻素及芝麻林素
Other Title
Determination of Sesamin and Sesamolin by Using HPLC with an Internal Standard
Journal
臺灣農業化學與食品科學
Journal Volume
51
Journal Volume
51
Journal Issue
2
Journal Issue
2
Pages
102-108
Start Page
102
End Page
108
Date Issued
2013-04
Author(s)
Abstract
芝麻中天然存在的木酚素（lignans）主要為芝麻素（sesamin）及芝麻林素（sesamolin），過去研究證實這些木酚素具有良好的生理功能。然而目前芝麻素及芝麻林素的定量研究並不完整，且尚未有公告的標準方法，因此文獻中使用的定量方法及所分析的數值差異頗大。本研究以萘（naphthalene）作為HPLC定量使用的內標準品，建立一種芝麻素及芝麻林素的定量方法，並作方法確效（validation）。本研究建立的定量方法回收率高，操作簡單、省時，每一樣品分析時間為25分鐘。芝麻素之定量極限（LOQ）為75 μg/L，芝麻林素之定量極限（LOQ）為150 μg/L。進一步以此方法對市售芝麻產品之芝麻素及芝麻林素含量進行分析，並與商品標示進行比較。本研究建立的定量方法可檢測芝麻保健食品中的有效成分，謹供學術界、業界及官方相關單位參考。
Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.
Subjects
芝麻
木酚素
芝麻素
芝麻林素
萘
Sesame seeds
Lignans
Sesamin
Sesamolin
Naphthalene
Publisher
臺灣農業化學會&臺灣食品科學技術學會
Type
journal article