Inhibition of TNF-α, IL-1α, and IL-1β by Pretreatment of Human Monocyte-Derived Macrophages with Menaquinone-7 and Cell Activation with TLR Agonists in Vitro
Journal
Journal of Medicinal Food
Journal Volume
19
Journal Issue
7
Pages
663-669
Date Issued
2016
Author(s)
Abstract
Circulatory markers of low-grade inflammation such as tumor necrosis factor-alpha (TNF-α), interleukin-1 alpha (IL-1α), and interleukin-1 beta (IL-1β) positively correlate with endothelial damage, atheroma formation, cardiovascular disease, and aging. The natural vitamin K2-menaquinone-7 (MK-7) added to the cell culture of human monocyte-derived macrophages (hMDMs) at the same time as toll-like receptor (TLR) agonists did not influence the production of TNF-α. When the cells were pretreated up to 6 h with MK-7 before treatment with TLR agonists, MK-7 did not inhibit significantly the production of TNF-α after the TLR activation. However, 30 h pretreatment of hMDMs with at least 10 μM of MK-7 effectively and dose dependently inhibited the proinflammatory function of hMDMs. Pretreatment of hMDMs with 10 μM of MK-7 for 30 h resulted in 20% inhibition of TNF-α production after lipopolysaccharide (LPS) activation (P < .05) and 43% inhibition after macrophage-activating lipopeptide (MALP) activation (P < .001). Pathogen-associated molecular pattern (PMPP) activation was inhibited by 20% with MK-7 pretreatment; however, this inhibition was not statistically significant. The 30 h pretreatment of a THP-1-differentiated monocyte cell line with MK-7 resulted in a dose-dependent downregulation of TNFα, IL-1α, and IL-1β gene expression as evaluated by RNA semiquantitative reverse transcription polymerase chain reaction (RT-PCR). MK-7 is able to modulate immune and inflammatory reactions in the dose-response inhibition of TNF-α, IL-1α, and IL-1β gene expression and protein production by the healthy hMDMs in vitro. ? 2016 Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition.
Subjects
cardiovascular; inflammation; MenaQ7 Crystals; menaquinone-7; TNF-a
SDGs
Other Subjects
farnoquinone; interleukin 1alpha; interleukin 1beta; lipopeptide; lipopolysaccharide; macrophage activating lipopeptide; menaquinone 7; pathogen associated molecular pattern; toll like receptor agonist; tumor necrosis factor alpha; unclassified drug; antiinflammatory agent; farnoquinone; IL1A protein, human; interleukin 1alpha; interleukin 1beta; lipopolysaccharide; toll like receptor; tumor necrosis factor; vitamin MK 7; Article; cell activation; cell viability; controlled study; cytokine production; down regulation; gene expression; human; human cell; immunomodulation; in vitro study; inflammation; macrophage; monocyte; priority journal; agonists; analogs and derivatives; antagonists and inhibitors; cell line; drug effects; genetics; macrophage; Anti-Inflammatory Agents; Cell Line; Gene Expression; Humans; Interleukin-1alpha; Interleukin-1beta; Lipopolysaccharides; Macrophages; Toll-Like Receptors; Tumor Necrosis Factor-alpha; Vitamin K 2
Type
journal article