Controlled Release of Curcumin from mPEG-PLGA Thermosensitive Hydrogel for Periodontal Tissue Repair
Date Issued
2014
Date
2014
Author(s)
Lin, Yan-Ting
Abstract
Periodontal disease is a chronic inflammatory disease and is caused by periodontal pathogens which destroy the periodontium including the gingival, periodontal ligament and alveolar bone. The treatment of periodontal disease is first to remove the plaque and dental calculus and then minocycline, a tetracycline antibiotics, is administered to the periodontal pocket for pharmacotherapy. However, the minocycline is not always effective on all pathogens, and abusing the antibiotics lead to the creation of resistant strains. Previous studies have reported that curcumin has a variety of biological activities, such as anti-inflammation properties, inhibition of osteoclastogenesis and matrix metalloproteinases. It seems that curcumin has a potential therapeutic role on the periodontal disease. Due to its extremely low solubility and poor stability lead to poor oral bioavailability. In this study, mPEG-PLGA thermosensitive hydrogel was used as a drug carrier of curcumin, and subjected the evaluation on periodontal tissue repair. The results suggest that curcumin-loaded mPEG-PLGA hydrogel has sol-gel transition property at 37℃, and curcumin can be sustainedly released from mPEG-PLGA hydrogel. In degradation test, mPEG-PLGA hydrogel retained 50% of its original weight at 37oC after four weeks. According to in vitro studies, both curcumin-loaded mPEG-PLGA hydrogel and mPEG-PLGA hydrogel have no cytotoxicity on rat gingival fibroblast cell (rGF), human osteosarcoma cell line (MG-63) and Mouse leukaemic monocyte macrophage cell line (RAW 264.7). In addition, curcumin-loaded mPEG-PLGA hydrogel can significantly decrease the expression of inflammatory factors such as IL-1β, IL-6 and TNF-α from RAW 264.7. In vivo studies show that curcumin-loaded mPEG-PLGA hydrogel can reduce the extent of alveolar bone resorption and decreases the inflammatory infiltrate in the gingival tissue. These outcomes suggest that curcumin-loaded mPEG-PLGA hydrogel has a potential therapeutic role on the periodontal disease.
Subjects
牙周病
薑黃素
溫感型水膠
發炎反應
齒槽骨溶蝕
SDGs
Type
thesis
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