生物時鐘調控甘藷葉部澱粉合成酵素基因表現之分子機制
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
王淑珍
DOI
922313B002100
Abstract
A starch granule-bound starch synthase I (GBSSI) gene is regulated by a circadian
clock in sweet potato leaves. In order to examine whether the promoter region is
responsible for controlling a circadian expression of the GBSSI gene, the sweet potato
GBSSI promoter was isolated and deleted to different lengths for functional analysis
with a GUS reporter gene in transgenic Arabidopsis plants. Nuclear run-on
transcriptional assays showed that the circadian control was regulated at the
transcriptional rate level, and de novo synthesized proteins were necessary for
controlling the rhythm. Promoter assays showed that the GBSSI promoter fragments
containing six I-boxes, two putative circadian regulation elements (CAANNNNATC)
and four circadian clock-associated 1 protein-binding sites (AATCT) maintained the
activity to induce the circadian expression of the GUS gene. Similar to the GBSSI in
sweet potato, GBSSI, soluble starch synthase and ADP-glucose pyrophosphorylase
genes in Arabidopsis leaves also exhibited a circadian rhythm. These results
suggested that common signals may exist in dicotyledonous plants to coordinate the
circadian expression of genes involved in the transitory starch synthetic pathway.
Subjects
Circadian clock
Granule-bound starch synthase
Ipomoea batatas
Starch
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
922313B002100.pdf
Size
907.94 KB
Format
Adobe PDF
Checksum
(MD5):bbf5433e2662f986bb5982a6ab540bec