Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate
Journal
Biochemical and Biophysical Research Communications
Journal Volume
301
Journal Issue
4
Pages
1062-1068
Date Issued
2003
Author(s)
Abstract
(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade. © 2003 Elsevier Science (USA). All rights reserved.
Type
journal article