Relationship between the volatile compounds profile and hepatoma inhibitory activity of the fermentation broth of Antrodia cinnamomea
Date Issued
2008
Date
2008
Author(s)
Huang, Tzu-Ning
Abstract
Antrodia cinnamomea is a folk medicine well known for its hepatoprotective, anti-inflammation function in Taiwan. Submerged fermentation is used to produce its bioactive material under controlled conditions to overcome its time consuming cultivation in solid state. During fermentation, there is an increase in the hepatoma inhibitory effect and the volatile compounds changes during each stage. The aim of this study is to investigate the correlation between the specific volatile compounds and bio-active components formed in due course of fermentation. In volatile compound analysis, PDMS/ DVB fiber was found to be most suitable fiber under the operating conditions of 60℃ for 30 minutes, to absorb volatile compounds of fermentation broth of A. cinnamomea. The GC-MS results indicated that the main volatile compounds were 1-octen-3-ol, linalool, methyl phenylacetate, nerolidol, γ-cadinene and 2,4,5-trimethoxybenzaldehyde. During fermentation it was observed that the fermentation broth have the highest inhibitory effect on 24th day. Also it was observed that among the various volatile compounds, there was a significant increase in concentration of 2,4,5-trimethoxybenzaldehyde in fermentation broth on the same day. Thus the volatile compound 2,4,5-trimethoxybenzaldehyde could be related to hepatoma inhibitory activity of fermentation broth, and can be used as a direct marker to decide harvesting time for the fermentation process.
Subjects
Antrodia cinnamomea
volatile compound
fermentation
Hep G2 cell
Solid Phase Microextraction (SPME)
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95641018-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):45199fc1412cd3d2899754fd083fac44