Molecular defense mechanism of tomato against bacterial wilt
Date Issued
2008
Date
2008
Author(s)
Chen, Yong-Yi
Abstract
Bacterial wilt (BW), caused by Ralstonia solanacearum, is a devastating vascular disease of many economically important crops worldwide. However, information on plant defense mechanism to the infection of this soil-borne bacterium is limited. In this study, virus-induced gene silencing (VIGS) was employed to decipher signaling pathways involved in tomato resistance to this pathogen. Firstly, factors involved in Tobacco rattle virus (TRV)-based VIGS system were optimized for efficient gene functional study in tomato. Spatial and temporal analysis showed that gene silencing in 9-day-old tomato plant initiates 5 days post inoculation (DPI) in stembases, 7 DPI in roots and 9 DPI in young leaves. Analysis involving multi-gene silencing revealed that a chimeric construction approach is more efficient as compared to mixing multiple Agrobacterium strains carrying individual target genes. By employing the optimized system, this study further revealed silenced salicylic acid-related signaling transduction pathways and mitogen-associated protein kinase cascades play role in tomato BW-resistance. This is the first report elucidating the intertwined defense network governing resistance to bacterial wilt in tomato. Finally, previous microarray analysis led to the identification of a group genes differentially expressed specifically in H7996. Using the VIGS system, this study further confirmed a few of these genes do play a role in tomato defense to R. solanacearum. Two of these genes, SlCLL and SlRLI2, were subjected to further characterization. Together these studies enlighten molecular mechanism of tomato BW resistance and genes involved.
Subjects
Solanum lycopersicum
Ralstonia solanacearum
SA
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