Characterization of thermally induced transitions of collagen using second harmonic generation (SHG) microscopy
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
5698
Date Issued
2005-08-16
Author(s)
Sun, Yen
Hsiao, Chih Yuan
Teng, Shu Wen
Lo, Wen
Tan, Hsing Yuan
Jan, Gwo Jen
Abstract
As a major component of the connective tissues, collagen fibers are responsible for various physiological functions inside the body. They provide support for the skin, partial focusing through the cornea, and coordinate movements via tendons, ligaments, and cartilages. In many medical procedures, thermal reorganization of the collagen structure is inevitable or desired. Therefore, the optimization of the therapeutic values of these procedures requires the characterization of thermal changes to collagen fibers. In this presentation, we use multiphoton microscopy to achieve this task. We will show that second harmonic generation (SHG) microscopy can characterize the thermally altered states of collagen and that they have potentials to be used in imaging applications in vivo.
Subjects
Collagen | Multiphoton microscopy | Second-harmonic generation | Thermal transition
Type
conference paper