Comparing the effect of diethylstilbestrol and estradiol on the expression of proline-rich acidic protein in mouse uterus
Date Issued
2004
Date
2004
Author(s)
HSIEH, TSAI-FU
DOI
zh-TW
Abstract
I compared the protein components in the uterine luminal fluid (ULF) collected from either the diethylstilbestrol (DES)-stimulate baby mouse or normal adult females at estrus stage. By the mass spectrometry, I identify 14- kDa protein to be proline-rich acidic protein (PRAP) that was undetectable in ULF of the DES-stimulated baby mice but appeared in ULF of adult in estrus stage.
In the natural estrous cycle, the mRNA level of PRAP appeared in proestrus, increased markedly in estrus and early metestrus and declined sharply in the late metestrus and diestrus to an almost undetectable level. PRAP mRNA was not detected in the uterus of immature mice. Immunohistochemical staining patterns of the estrous uterine section showed that PRAP was immunolocaliezed in glandular and epithelial cells of endometrium. Although treatment of baby mice with DES for consecutive three days did not stimulated the PRAP expression in the uterine an injection of estradiol (E2) alone or E2 together with progesterone (P4) for consecutive three days stimulated of the PRAP expression. Six days after the first injection of E2 together with P4, the PRAP mRNA increased remarkedly. Neonatal DES exposure did not coursed a reduction but prepubertal DES exposure decreases remarkedly in the uterine expression of PRAP when the animals were sexually matured.
Subjects
多脯胺酸酸性蛋白
proline-rich acidic protein
Type
other
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