Determination of mitochondrial functions and damage in kidney in female LeeSung minipigs with a high-fat diet-induced obesity
Journal
Archives of Physiology and Biochemistry
Journal Volume
129
Journal Issue
6
Start Page
1289
End Page
1297
Date Issued
2021-08-02
Author(s)
Miao-Ju Chien
Wong, Shiu-Chung
Chiang, Chun-Hsien
Mersmann, Harry J.
Abstract
The purpose of this study was to investigate the nexus between mitochondrial function and kidney injury by using a dietary-induced obese minipig model. Female Lee-Sung minipigs feeding a high-fat diet (HFD) for 6 months exhibited obesity, hyperglycaemia and dyslipidemia. HFD elevated the levels of plasma biomarkers related to renal injury, including symmetric dimethylarginine, creatinine and urea nitrogen. An extensive structural change in tubules and glomeruli was observed in HFD-fed pigs. A great amount of triacylglycerol was accumulated in HFD kidney compared to control kidney, whereas a reduction of ATP level and antioxidant capacity were exhibited in HFD kidney. Moreover, HFD altered the expressions of mitochondrial-related protein in renal cortex. To conclude, long-term HFD feeding to Lee-Sung minipigs induced obesity and kidney injury accompanied by abnormal mitochondrial functions in the renal cortex, suggesting an interrelationship with renal disease progression.
Subjects
Biogenesis
high fat diet
mitochondrial dynamic
renal damage
Type
journal article