Expression and characterization of two STAT isoforms from Sf9 cells
Resource
Developmental & Comparative Immunology, 32(7), 814-824
Journal
Developmental & Comparative Immunology
Pages
814-824
Date Issued
2008
Date
2008
Author(s)
Yeh, Maw-Sheng
Cheng, Chia-Hsiung
Chou, Chih-Ming
Hsu, Ya-Li
Chu, Cheng-Ying
Chen, Gen-Der
Chen, Shui-Tsung
Chen, Guang-Chao
Huang, Chang-Jen
Abstract
In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
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