Effect of extracts from selected edible Myrtaceae plants on the alleviation of insulin resistance in mouse liver FL83B cells
Date Issued
2010
Date
2010
Author(s)
Chang, Wen-Chang
Abstract
As advanced medical technology and improved living standard increased life expectancy, chronic diseases have become a major threat to the health of people. Diabetes is one of the fastest growing chronic diseases. Guava (Psidium guajava Linn.) is belonged to the Myrtaceae plants and it has many references confirming its functions to alleviate diabetic symptoms. In this study, I will search others edible Myrtaceae plants in Taiwan, included Syzygium samarangense (Blume) Merrill et L.M. Perry, Psidium guajava Linn., Syzygium jambo(L.) Alston, Syzygium cumini Linn., screening extracts for potential of anti-hyperglycemic activity and search the active compound to enhance glucose uptake.
First, Myrtaceae plants are extracted by different solvents (water and ethanol) to test the viability of the mouse liver cell FL83B. Safety dosage of the extracts of Myrtaceae plant is also evaluated. Secondly, the research will investigate the effect of the Myrtaceae plants extract (100μg/mL) on glucose uptake and evaluate on amelioration of carbohydrate metabolism in TNF-α-induced insulin resistant mouse liver FL83B cells. Finally, the best performing Myrtaceae plants to increase glucose uptake in the insulin resistant cell was analyzed by column chromatography, and the active components of the Myrtaceae plants which could decrease the blood sugar would be isolated and purified.
Based on the results of cell viability test, majority of the Myrtaceae plants have no cell toxicity in concentration of 100μg/ml, and there were ten of ethanol extracts with no cell toxicity in a lower concentration (50μg/mL). Results of the glucose uptake ability in the insulin resistant cell modular indicated the group with the highest improvement is the water extract of unripe pink wax apple fruit. Compared with the control group (TNF-α treated group), the water extract of unripe pink wax apple fruit could increase the glucose uptake ability by 21%. Next is the water extract and the ethanol extract of unripe fruit of Syzygium cumini Linn., the percentage increase of the glucose uptake were 18.32% and 17.23% respectively.
In conclusion, the water extracts of unripe wax apple fruit was collected for column chromatography. After several elutions, the high polarity fraction 1 at the concentration of 12.5μg/mL, had similar results as the water extract of unripe pink wax apple fruit at 100 μg/mL, in improving glucose uptake in the insulin resistant cell. The A partition was speculated to be the active component of the water extract of unripe pink wax apple fruit in decreasing blood sugar. The structure of the unknown extract is being identified.
Subjects
Myrtaceae plant
insulin resistance
glucose uptake
unripe pink wax apple fruit
SDGs
Type
thesis
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