Population structure of albacore (Thunnus alalunga) in the Northwestern Pacific Ocean inferred from mitochondrial DNA
Date Issued
2008
Date
2008
Author(s)
Wu, Georgiana Cho-Chen
Abstract
Albacore (Thunnus alalunga) is a highly migratory cosmopolitan fish commonly distributed throughout tropical, subtropical and temperate areas of all oceans including the Mediterranean Sea. Due to its commercial and economic importance, a better understanding of its genetic structure is essential to an effective fishery management. In the current study, population structure of albacore in the Northwestern Pacific Ocean was investigated using mitochondrial DNA control region (D-loop) sequence data specifically of the first hypervariable region (HVR-1).A total of 175 individuals were caught and sampled from three regions in the Northwestern Pacific Ocean (Taiwan, Japan and Hawaii). Total DNA of each sample was isolated and purified. With the use of specific primers, the entire mtDNA control region was amplified through Polymerase Chain Reaction. The mtDNA were aligned using ClustalX and 168 haplotypes were revealed. Neighbor-joining tree based on the Kimura 2-parameters model was constructed using MEGA with 1000 bootstrap replicates. The reconstructed neighbor-joining phylogeny tree based on sequencing data of these 175 samples suggested that albacore haplotypes in these three sampling regions can be divided into two main clades (Clade I and Clade II) with one clade (Clade I) further sub-divided into two lineages (Lineage I and Lineage II). Hierarchical AMOVA tests and pairwise FST analysis showed that albacore tuna in the Northwestern Pacific Ocean constituted a single stock with no significant differences in geographic distributions.
Subjects
Albacore (Thunnus alalunga)
population genetics
mitochondrial DNA
control region
Northwestern Pacific Ocean
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