Composite Microstructural Substrate Induces Cell Migration
Date Issued
2011
Date
2011
Author(s)
Chang, Wei-Jen
Abstract
The thesis used the laboratory-designed composite substrate microstructure to study the cell morphology and behavior.
This composite substrate device offers a homogeneous chemical property of 2D and 3D environment, and the inner geometric and microstructural design can be easy and precious to manipulate.
First, we used the finite element method to simulate the mechanical property of composite substrate and atomic force microscopy(AFM) to measure the effective modulus of composite substrate.
There are four research topics in the thesis, including effect of collagen thickness and spacing distance between micropillars on human mesenchymal stem cells (hMSCs) migration and morphology, and effect of three-dimension environment on hMSCs migration and morphology, and effect of composite substrate microstructure on anterior cruciate ligament cells (ACL) migration and morphology.
In our study, most cells migrated toward the composite thin gel on the micropillars, and this implies cells seem like to have potential to sense the underlying structure of our composite substrate. In addition, different cell types have different sensitivity for the underlying structure of our composite substrate.
Subjects
Cell migration
Stiffness
Type
thesis
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