Development of Pulse Sequences for Biological Solid-State NMR Based on Magnetic Dipole-Dipole Interaction
Date Issued
2011
Date
2011
Author(s)
Lee, Hsin-Kuan
Abstract
This work contains the development of pulse sequences in solid-state NMR and dihedral angle determination of biological solids based on magnetic dipole-dipole interaction. Chapter 1 gives a review of using solid-state NMR to obtain the structural information for biological solids. Chapter 2 introduces the theoretical background for solid-state NMR. The main thesis contains three topics. First, we show by a detailed analysis that a set of internal selection rules does exist for many basic elements of pulse sequences. And we have derived the symmetry arguments to rationalize the observation that the C-REDOR pulse sequence can suppress homonuclear dipole-dipole interaction (Chapter 3). The concept of the internal symmetry has further applied to increase the efficiency of C-REDOR (Chapter 4). Second, we design a constant-time sequence composed of DRAMA-based pulses for homonuclear dipolar dephasing (Chapter 5 and 6). Third, we introduce an approach for the dihedral angle determination of biological samples. In this part, we discuss the psi angle determination by correlation of the 13C(alpha)-1H(alpha) and the 13C(alpha)-14N dipolar tensors within a single labeled residue (Chapter 7).
Subjects
solid-state NMR
pulse sequence
dipole-dipole interaction
homonuclear dipolar recoupling
heteronuclear dipolar recoupling
distance measurement
psi angle measurement
Type
thesis
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