Bead-String-Shaped DNA Nanowires with Intrinsic Structural Advantages and Their Potential for Biomedical Applications
Journal
ACS Applied Materials and Interfaces
Journal Volume
12
Journal Issue
3
Pages
3341-3353
Date Issued
2020
Author(s)
Abstract
Due to high programmability and good biocompatibility, DNA has been recognized as a powerful building block for engineering of sophisticated nanostructures for different purposes. Herein, we present the first example of a bead-string-shaped DNA nanowire (BS-nanow) with long-range structural order for in vivo bioimaging and targeted drug delivery. BS-nanow is assembled from DNA tetrahedron units with precise nanometer-scale spatial control, capable of accommodating chemotherapeutic agents with high payload capacity (1204 binding sites) as well as possessing a 60-fold enhanced binding affinity for target cells. Confocal fluorescence imaging and in vivo experiments on CEM subcutaneous tumor-bearing mice show that specific bioimaging of living cells and even systemic delivery of drugs into internal tumor organs and tissues were accomplished, thereby achieving an efficient inhibition of tumor growth in the xenograft model without systemic toxicity. BS-nanow's show potential in vivo applications in accurate diagnosis and targeted therapy for human cancer. Copyright ? 2019 American Chemical Society.
Subjects
bead-string-shaped DNA nanowire; DNA tetrahedron; drug loading; multivalent aptamers; targeted drug delivery
SDGs
Other Subjects
Binding energy; Binding sites; Biocompatibility; Controlled drug delivery; Diagnosis; DNA; Fluorescence imaging; Geometry; Mammals; Medical applications; Nanowires; Tumors; Aptamers; Biomedical applications; Chemotherapeutic agents; Confocal fluorescence imaging; Drug loading; In-vivo experiments; Inhibition of tumor growth; Structural advantage; Targeted drug delivery; antineoplastic agent; DNA; drug carrier; nanowire; animal; Bagg albino mouse; chemistry; devices; drug delivery system; human; male; mouse; neoplasm; procedures; tumor cell line; Animals; Antineoplastic Agents; Cell Line, Tumor; DNA; Drug Carriers; Drug Delivery Systems; Humans; Male; Mice; Mice, Inbred BALB C; Nanowires; Neoplasms
Type
journal article