Identification and molecular characterization of dPTPMEG-interacting proteins
Date Issued
2011
Date
2011
Author(s)
Lin, Yu-Ling
Abstract
Protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) reciprocally regulate protein tyrosine phosphorylation, and is involved in various cellular processes, including cell cycle, proliferation, differentiation and cell movement. Classical PTPs can be classified into receptor-like PTPs (RPTPs) and non-transmembrane PTPs (NTPTPs). Although many Drosophila RPTPs have been found to act during neurogenesis, the roles of NTPTPs in development remain unclear. In this study, we focused on the dPTPMEG, one of the Drosophila classical non-transmembrane PTPs (NTPTPs), which has been reported to play a role in the maintenance of axon projection of Drosophila mushroom body. To further explore the function of dPTPMEG, the yeast two-hybrid screen was performed to isolate dPTPMEG-interacting proteins from Drosophila embryonic cDNA library. Two proteins have been identified that can potentially interact with dPTPMEG. The interaction between dPTPMEG and these two proteins was further confirmed by in vitro GST pull-down assays and in vivo co-immunoprecipitation experiments. We further investigated their genetic interactions in Drosophila developing wings. The developmental roles of these interactions will be further investigated.
Subjects
Yeast two-hybrid screen
tyrosine phosphatase
dPTPMEG
GST-pull down assay
co-Immunoprecipitation
Type
thesis
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