The association of single nucleotide polymorphisms in the MMP-9 genes with susceptibility to acute primary angle closure glaucoma in Taiwanese patients
Journal
Molecular Vision
Journal Volume
12
Pages
1223-1232
Date Issued
2006
Author(s)
Chiang T.-H.
Shih Y.-F.
SHAO-CHUN LU
Lin L.L.-K.
Shieh J.-W.
Samples J.R.
Hung P.-T.
Abstract
Purpose: To study the relationships between single nucleotide polymorphisms (SNPs) of extracellular matrix, matrix metalloproteases (MMPs), tissue inhibitors of MMPs, and other glaucoma-associated genes and acute primary angle closure glaucoma (PACG). Methods: We extracted DNA samples from 78 adult patients with acute PACG and 86 control subjects to study the relationships between these specific genes and acute PACG. Genotyping was performed for 35 genes by the GenomeLab SNPstream genotyping system after PCR amplification of chromosomal DNA. The association between these genetic polymorphisms and risk of primary PACG was estimated by χ2 and logistic regression. Results: The genotyping success rate was 99%. Genotyping for the MMP9 site (rs2664538) was significantly different between the two groups (p=0.000001) and the odds ratio was 2.586 (95% CI: 1.715-3.898, p<0.00001). However, there were no associations of SNPs to other genes in patients with acute PACG. Conclusions: Our results reveal that SNP rs2664538, which is located at the MMP9 gene, is likely to be associated with acute PACG. ? 2006 Molecular Vision.
SDGs
Other Subjects
adenine; arginine; gelatinase B; glutamine; guanine; matrix metalloproteinase; tissue inhibitor of metalloproteinase; adult; aged; amino acid substitution; article; chi square test; chromosome 20q; closed angle glaucoma; codon; controlled study; DNA determination; extracellular matrix; female; genetic association; genetic risk; genetic susceptibility; genotype; homozygosity; human; logistic regression analysis; major clinical study; male; nucleic acid base substitution; polymerase chain reaction; priority journal; single nucleotide polymorphism; statistical significance; Taiwan; Acute Disease; Aged; Asian Continental Ancestry Group; Female; Genetic Predisposition to Disease; Genotype; Glaucoma, Angle-Closure; Homozygote; Humans; Logistic Models; Male; Matrix Metalloproteinase 9; Middle Aged; Odds Ratio; Polymorphism, Single Nucleotide; Taiwan
Type
journal article