Ulva prolifera polysaccharide exerts anti-obesity effects via upregulation of adiponectin expression and gut microbiota modulation in high-fat diet-fed C57BL/6 mice
Journal
Journal of Food and Drug Analysis
Journal Volume
30
Journal Issue
1
Pages
46-61
Date Issued
2022
Author(s)
Abstract
Obesity is characterized by metabolic disorder and accompanying an altered and less diverse gut microbiota composition during a fat-enriched diet. Recent studies indicated that sulphated polysaccharide prevents high-fat diet (HFD) induced obesity, reduces metabolic disorder, and restores the gut microbiota. However, there are few studies about Ulva prolifera polysaccharide (UPP) may induce anti-obesogenic effects. Therefore, the present study investigates the enzymatic extracted UPP effects in HFD-fed mice. The results showed that UPP considerably slowed down the HFDinduced weight gain and improved metabolic disorders in HFD-fed mice. Notably, the effects were associated with lower body weight gain, reduced adipose tissue hypertrophy, triglyceride concentration in liver and systemic low-grade inflammation, and improved fasting blood glucose. Moreover, our result reveals that UPP may elevate the expression of AMPK via adiponectin activation. Interestingly, we found that UPP may induce PPARa agonist to enhance b-oxidation since the elevation of CPT-1 and PPARa expression simultaneously. Meanwhile, gut microbiota analysis revealed UPP promoted the growth of Parasutterella, Feacalibaculum, and Bifidobacterium, and reduced the abundance of Acetatifactor, Tyzerella, Ruminococcus_1, and Desulfovibrio. The changes in microbiota may have a positively correlated effect on improving obesity and metabolic abnormalities. UPP may prevent HFD-induced obesity and associated metabolic diseases, as well as modulate the composition of gut microbiota to facilitate the growth of probiotics. © 2022, Taiwan Food and Drug Administration. All rights reserved.
Subjects
Gut microbiota; Metabolic disorder; Obesity; Sulphated polysaccharide; Ulva prolifera
SDGs
Other Subjects
adiponectin; peroxisome proliferator activated receptor alpha; polysaccharide; adverse event; animal; C57BL mouse; intestine flora; lipid diet; metabolism; mouse; obesity; Ulva; upregulation; Adiponectin; Animals; Diet, High-Fat; Gastrointestinal Microbiome; Mice; Mice, Inbred C57BL; Obesity; Polysaccharides; PPAR alpha; Ulva; Up-Regulation
Type
journal article