與胃癌、消化性潰瘍及宿主免疫力相關的特異性幽門螺旋桿菌菌株蛋白之研究
Date Issued
2004
Date
2004
Author(s)
楊智欽
DOI
922314B002142
Abstract
Recent studies strongly suggest that chronic infection with H. pylori is tightly
associated with chronic gastritis, peptic ulcer, and gastric carcinoma. However, only a
part of infected people develop signs and symptoms of gastric pathology. Thus, both
host and microbial factors may lead to different outcomes of infection. Perhaps the
greatest concern with regard to infection with H. pylori is the increased risk for the
development of gastric cancers in adulthood. This is particularly relevant because
infection dating from childhood appears to enhance the risk of carcinogenesis.
Recently, in a continuous epidemiological study for the H. pylori infection in the
adolescence and children, we have found a steady proportion of these young
individuals year by year having the ability to produce spontaneous clearance of the H.
pylori infection. Meanwhile, in another studies, we also have identified two candidate
proteins, one from the patients with gastric cancer, the other from the patients with
gastric MALToma. In a pilot study, however, we have found many protein bands
which demonstrated on a 1-D SDS-PAGE can be separated more clearly to several
protein spots by 2-D gel electrophoresis plus immunoblotting detection. In this project,
using technology of proteomics, we have found 1) whole-cell proteins: Three protein
spots on the 2-DE gel were found in the H. pylori isolated from the patients with
duodenal ulcer. These 3 protein spots were not found in the strains related to the
patients with gastric ulcer and gastric cancer. Two protein spots on the 2-DE gel were
specifically associated with chronic gastritis only. There were 2 protein spots on the
2-DE gel of H. pylori isolated from the patients with gastric cancer. These 2 protein
spots were not noted in the strains related to the patients with other condition. 2)
Secreted proteins: Two proteins spots were found from the H. pylori strains
specifically related to the patients with chronic gastritis only. Meanwhile, there were
one protein spot on the 2-DE gel of H. pylori isolated from the patients with gastric
cancer. This protein spot was not noted in the strain associated with other disease
patterns. This study will provide further insight not only in understanding of
pathogenic mechanism in H. pylori infection, but also the direction of developing new
therapies or vaccines.
Subjects
Helicobacter pylori
major antigen
mouse animal model
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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