Synbiotic combination of djulis (Chenopodium formosanum) and lactobacillus acidophilus inhibits colon carcinogenesis in rats
Journal
Nutrients
Journal Volume
12
Journal Issue
1
Date Issued
2020
Author(s)
Abstract
Djulis is a functional grain containing prebiotic dietary fiber, which has an anti-cancer potential. This study examined the preventive effect of djulis alone or in combination with Lactobacillus acidophilus on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) and dextran sulfate sodium (DSS). Rats were divided into five groups and fed B (AIN-93G, blank), C (AIN-93G, control), D (10% djulis), DLA (10% djulis plus 5 × 106 cfu L. acidophilus/g), and DHA (10% djulis plus 5 × 107 cfu L. acidophilus/g) diets, respectively. All rats except for those in group B received three doses of DMH (40 mg/kg) by intraperitoneal injection and 3% DSS in drinking water. After 10 weeks of feeding, the colon was analyzed for precancerous lesions and biomarkers. DMH and DSS treatment induced aberrant crypt foci (ACF), especially in the distal colon. D, DLA, and DHA significantly reduced the numbers of total ACF, sialomucin-producing ACF (SIM-ACF), and mucin-depleted foci (MDF) in the distal colon compared to C. Additionally, DLA and DHA further downregulated the expressions of proliferating cell nuclear antigen (PCNA) and cyclooxygenase-2 (COX-2) and regulated apoptosis-related proteins. These results suggest that synbiotic combination of djulis and L. acidophilus shows the best inhibitory effect on colon carcinogenesis via regulation of proliferative, inflammatory, and apoptotic pathways. ? 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Apoptosis; Colon cancer; Djulis; Lactobacillus acidophilus; Synbiotics
SDGs
Other Subjects
1,2 dimethylhydrazine; apoptosis regulatory protein; caspase 3; cycline; cyclooxygenase 2; dextran sulfate; la 5; prebiotic agent; probiotic agent; protein Bax; protein bcl 2; sialomucin; synbiotic agent; 1,2 dimethylhydrazine; dextran sulfate; synbiotic agent; aberrant crypt focus; animal experiment; animal model; antineoplastic activity; apoptosis; Article; body weight; cancer inhibition; cecum; cell proliferation; chemoluminescence; Chenopodium; Chenopodium formosanum; colon carcinogenesis; colony forming unit; controlled study; descending colon; diet supplementation; dietary fiber; down regulation; feces analysis; food intake; gene expression; Lactobacillus acidophilus; nonhuman; polyacrylamide gel electrophoresis; protein expression; rat; animal; carcinogenesis; colon tumor; drug effect; gene expression regulation; inflammation; 1,2-Dimethylhydrazine; Animals; Apoptosis; Carcinogenesis; Cell Proliferation; Chenopodium; Colonic Neoplasms; Dextran Sulfate; Gene Expression Regulation; Inflammation; Lactobacillus acidophilus; Rats; Synbiotics
Type
journal article