Signal Amplified Gold Nanoparticles for Cancer Diagnosis on Paper-Based Analytical Devices
Journal
ACS Sensors
Journal Volume
3
Journal Issue
1
Pages
174--182
Date Issued
2018-01
Author(s)
Jia-Yu Huang
Hong-Ting Lin
Tzu-Heng Chen
Chung-An Chen
Huan-Tsung Chang
Chien-Fu Chen
Abstract
In this work, we report a highly sensitive colorimetric sensing strategy for cancer biomarker diagnosis using gold nanoparticles (AuNPs) labeled with biotinylated poly(adenine) ssDNA sequences and streptavidin-horseradish peroxidase for enzymatic signal enhancement. By adopting this DNA-AuNP nanoconjugate sensing strategy, we were able to eliminate the complicated and costly thiol-binding process typically used to modify AuNP surfaces with ssDNA. In addition, different antibodies can be introduced to the AuNP surfaced via electrostatic interactions to provide highly specific recognition sites for biomolecular sensing. Moreover, multiple, simultaneous tests can be rapidly performed with low sample consumption by incorporating these surface-modified AuNPs into a paper-based analytical device that can be read using just a smartphone. As a result of these innovations, we were able to achieve a detection limit of 10 pg/mL for a prostate specific antigen in a test that could be completed in as little as 15 min. These results suggest that the proposed paper platform possesses the capability for sensitive, high-throughput, and on-site prognosis in resource-limited settings. ? 2017 American Chemical Society.
Subjects
cancer diagnosis; colorimetric assay; gold nanoparticles; paper-based analytical device; signal amplification
Other Subjects
Analytic equipment; Antigens; Colorimetry; Diseases; Fiber optic sensors; Metal nanoparticles; Paper; Proteins; Biomolecular sensing; Cancer diagnosis; Colorimetric assays; Horse-radish peroxidase; Paper-based analytical devices; Prostate specific antigen; Signal amplifications; Specific recognition; Gold nanoparticles; antibody; gold; horseradish peroxidase; metal nanoparticle; single stranded DNA; streptavidin; tumor marker; animal; chemistry; colorimetry; human; neoplasm; paper; procedures; smartphone; Animals; Antibodies; Biomarkers, Tumor; Colorimetry; DNA, Single-Stranded; Gold; Horseradish Peroxidase; Humans; Metal Nanoparticles; Neoplasms; Paper; Smartphone; Streptavidin
Type
journal article