Capillary Electrophoresis Electrospray Mass Spectrometry of Perfluorocarboxylic acids
Date Issued
2007
Date
2007
Author(s)
Shen, Shang-Yu
DOI
zh-TW
Abstract
Perfluoroalkylcarboxylates and perfluoroalkyksulfonates are popular fluorinated surfactants. Generally, the detection of these compounds was based on LC-MS technique. In this study, we try to use CE-MS as the tool to develop a rapid and sensitive method for the analysis of perfluorocompounds(PFCs). First, the low-flow interface was tested and ammonium acetate was used as the running buffer. Factors which may influence separation efficiency includes pH value, buffer concentration and organic solvents were studied. By varying these factors, we could get a optimized separation condition for PFCs analysis. However, phosphate buffer was reported to give better separation than acetate buffer in CE-UV. Unfortunately, phosphate buffer is shown to cause interference or suppress analyte signal. A liquid junction-low flow CE-MS interface was studied to alleviate these problems. In this design, phosphate buffer was placed in the liquid-junction reservoir. The mobility of EOF and phosphate ions needs to be controlled. At a concentration of 15mM ammonium phosphate in 50% MeOH, pH=8.3, the CE-MS interface demonstrated its utility to prevent phosphate ions entering the MS ionization source.
Subjects
毛細管電泳
質譜
全氟化合物
Electrophoresis
Mass Spectrometry
Perfluorocarboxylic acids
Type
thesis
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