In situ UV-crosslinked Electrospun Gelatin Fibers for Tissue Engineering
Date Issued
2012
Date
2012
Author(s)
Lin, Wei-Han
Abstract
Electrospun fibers fabricated by natural polymer usually consisted of high cell adhesion ability with many beneficial characteristics, including their biocompatibility and biodegradability. However, weak mechanical property and high dissolution property are some of the drawbacks for natural polymer in limiting its application in electrospun fiber for biomedical usage. In addition, the biological toxicity was noted during the crosslinkage process of electrospun fibers remained a crucial problem in current research with either precipitation of toxic substances after crosslinkage or inefficient methods for removing them. Besides, the commonly used two-step crosslinking method (crosslinked after fiber fabrication) may cause un-even crosslinking of interior and exterior of the electrospun scaffolds. In this research, UV crosslinker poly(acrylic acid)-g-azide (PAA-Az) combined with an in situ crosslinking method was used for crosslinkage of gelatin to provide a low biological toxic route in fabricating electrospun fibers with high fibroblast proliferation. Hydroxyapatite (HAp) was also mixed in the solution for fabrication of the crosslinked fibers, resulting in high mineralization of osteoblasts. Finally, HAp, PEI-RGD, and BMP-2 were mixed in the solution for fabrication crosslinked electrospun gelatin fibers, resulting in high cell proliferation and mineralization of mesenchymal stem cells.
As result, gelatin electronspun fibers crosslinked by PAA-Az exhibited higher mechanical strength and glass transition temperature in compared to un-crosslinked gelatin. When comparing gelatin electrospun fibers crosslinked by glutaraldeyde, low cell toxicity was observed with enhancement in fibroblast adhesion for PAA-Az crosslinked gelatin fibers. In addition, when blending HAp in the solution, higher mineralization was also observed in osteoblasts on PAA-Az crosslinked gelatin fibers in compared to crosslinked gelatin fibers without HAp. Finally, PEI-RGD incorporation indeed enhance 3A6 cell attachment and proliferation. HAp incorporation increase the calcium deposited amount of 3A6 cell seeded on the electrospun gelatin fibers. The research presents potential of the in situ PAA-Az crosslinking method in the application of natural and high dissoluble polymeric electrospun fibers for tissue engineering.
Subjects
electrospinning
UV-crosslinking
gelatin
azide
tissue engineering
Type
thesis
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