A new mosaic disease of Amazon lily in Taiwan
Resource
Plant Pathology 53 (2): 240
Journal
Plant Pathology
Journal Volume
53
Journal Issue
2
Pages
-
Date Issued
2004
Date
2004
Author(s)
Hu, W. C.
Abstract
Mosaic symptoms were observed in leaves of Amazon lily (Eucharis grandiflora) grown commercially in Taiwan. Leaf extracts caused necrotic local lesions when mechanically inoculated onto leaves of Chenopodium amaranticolor, Chenopodium quinoa and Gomphrena globosa, whereas seedlings of Nicotiana benthamiana showed a systemic mild mosaic. Transmission electron microscopy showed flexuous rods, approximately 700–780 nm long, in leaf extracts. In indirect ELISA, plants with symptoms tested positive using a monoclonal antibody specific to the potyvirus group (Agdia Inc., Elkhart, IN, USA). Two potyviruses have been reported to infect Amazon lily: Amazon lily mosaic virus (ALiMV) (Terami et al., 1993) and Hippeastrum mosaic virus (HiMV) (Jayasinghe & Dijkstra, 1979). Based on symptomatology, the virus isolate resembled ALiMV, as this causes a systemic mosaic in N. benthamiana, whereas HiMV does not infect this host (Brunt et al., 1996). Therefore, the virus was tentatively identified as ALiMV, although the presence of previously uncharacterized potyviruses cannot be excluded. The 3′-terminal region of ALiMV was amplified by RT-PCR from a total RNA extract from diseased plants using a potyvirus-specific degenerate forward primer (5′-GGBAAYAATAGTGGNCAACC-3′) and an oligo-dT reverse primer. A 1·6-kb amplicon was cloned into pGEM-T® Easy Vector (Promega, Madison, WI, USA), sequenced and then analysed using the GCG program of Wisconsin Package Version 10·3 (Accelrys Inc., San Diego, CA, USA). When compared with 22 potyviruses, the deduced amino acid sequence of the coat protein showed 40% identity to Dasheen mosaic virus (DsMV) and 55% identity to Ornithogalum mosaic virus (OrMV).
SDGs