Advancing Biocapture Substrates via Chemical Lift-Off Lithography
Journal
Chemistry of Materials
Journal Volume
29
Journal Issue
16
Pages
6829--6839
Date Issued
2017-08
Author(s)
Huan H. Cao
Nako Nakatsuka
rew C. Serino
Sarawut Cheunkar
Hongyan Yang
Paul S. Weiss
Anne M.
rews
Abstract
Creating small-molecule-functionalized platforms for high-throughput screening or biosensing applications requires precise placement of probes on solid substrates and the ability to capture and to sort targets from multicomponent samples. Here, chemical lift-off lithography was used to fabricate large-area, high-fidelity patterns of small-molecule probes. Lift-off lithography enables biotin–streptavidin patterned recognition with feature sizes ranging from micrometers to below 30 nm. Subtractive patterning via lift-off facilitated insertion of a different type of molecule and, thus, multiplexed side-by-side placement of small-molecule probes such that binding partners were directed to cognate probes from solution. Small molecules mimicking endogenous neurotransmitters were patterned using lift-off lithography to capture native membrane-associated receptors. We characterized patterning of alkanethiols that self-assemble on Au having different terminal functional groups to expand the library of molecules amenable to lift-off lithography enabling a wide range of functionalization chemistries for use with this simple and versatile patterning method.
Type
journal article