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The Impacts of Dietary Iron on Rat Intestinal Microbiota
Date Issued
2014
Date
2014
Author(s)
Chang, Yueh-Hsien
Abstract
It''s been very long time since the intriguing relationship between intestinal microbiota and human was established. Microbes not only make food tasty but also threaten our life. Recently, due to the advanced technology and knowledge of microbiota, many potential effects was speculated, examined, and applied, such as the nutritional benefits of probiotics. However, the interaction of host and microbiota is pretty complicated and involved in a number of factors. Therefore, the present study was to examine the role of iron in the succession of intestinal microbes.
In this study, weanling rats were fed an iron-deficient diet for 22 days and then repleted with iron (either organic form which was a commercial beverage fortified with sodium ferrous citrate or inorganic form which was ferrous sulphate) for 15 days. The alteration of rat fecal and cecal bacterial number was measured by real-time qPCR technique, and the iron content of cecum was measured by wet ashing method. Correlation analyses were also conducted between these two results.
First of all, cecal iron in iron sufficient group was significantly higher than the experimental groups; and no significant difference was found in these groups. It showed that exhausted cecal iron was still not yet restored even after the restore of Hb level. Then, qPCR results showed that no sigdifference was observed in the number of total bacteria between control group and experimental groups. Enterobacteriaceae were increased and decreased during the period of iron depletion and repletion respectively. The decrease in Lactobacillus / Leuconostoc / Pediococcus group and Bifidobacterium were slowed down during iron depletion. In contrast, the decreases in Roseburia spp. / E. rectale were restored during iron repletion. Slightly effects of organic or inorganic iron on Enterobacteriaceae and Bifidobacterium were observed. Finally, the results of correlation analyses indicated a positive correlation between iron content and total bacteria but a negative correlation between iron content and Enterobacteriaceae. Slightly negative correlation between iron content and Lactobacillus / Leuconostoc / Pediococcus group was also observed.
The impacts of didetary iron on certain bacteria, which possess special capability for iron, were discovered in this study. Therefore, further studies are still needed to examine the consequences caused by these bacteria during this process. In the future, further studying the impacts of dietary iron on human intestinal microbiota should lead to clinical implications.
In this study, weanling rats were fed an iron-deficient diet for 22 days and then repleted with iron (either organic form which was a commercial beverage fortified with sodium ferrous citrate or inorganic form which was ferrous sulphate) for 15 days. The alteration of rat fecal and cecal bacterial number was measured by real-time qPCR technique, and the iron content of cecum was measured by wet ashing method. Correlation analyses were also conducted between these two results.
First of all, cecal iron in iron sufficient group was significantly higher than the experimental groups; and no significant difference was found in these groups. It showed that exhausted cecal iron was still not yet restored even after the restore of Hb level. Then, qPCR results showed that no sigdifference was observed in the number of total bacteria between control group and experimental groups. Enterobacteriaceae were increased and decreased during the period of iron depletion and repletion respectively. The decrease in Lactobacillus / Leuconostoc / Pediococcus group and Bifidobacterium were slowed down during iron depletion. In contrast, the decreases in Roseburia spp. / E. rectale were restored during iron repletion. Slightly effects of organic or inorganic iron on Enterobacteriaceae and Bifidobacterium were observed. Finally, the results of correlation analyses indicated a positive correlation between iron content and total bacteria but a negative correlation between iron content and Enterobacteriaceae. Slightly negative correlation between iron content and Lactobacillus / Leuconostoc / Pediococcus group was also observed.
The impacts of didetary iron on certain bacteria, which possess special capability for iron, were discovered in this study. Therefore, further studies are still needed to examine the consequences caused by these bacteria during this process. In the future, further studying the impacts of dietary iron on human intestinal microbiota should lead to clinical implications.
Subjects
營養
鐵
膳食
腸道
微生物
Type
thesis
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