Simulation and Analysis of Cellular Labeling with Gold Nanoparticles
Date Issued
2008
Date
2008
Author(s)
Tsai, Hung-Hsin
Abstract
Surface plasmon resonance (SPR) is a phenomenon of free electrons on the surface of metal coupled to the electromagnetic excitations exhibiting coherent collective oscillations. The optical properties of SPR of metal are sensitive to particle size, shape and the surrounding medium. The SPR of metal nanoparticles have special optical properties that can be applied for biomedical applications; nanoparticles can be conjugated with anti-epidermal growth factor receptor (anti-EGFR) antibodies that bind specifically to the cells due to the overexpressed EGFR on the cytoplasmic membrane of the cancerous cells. In this research, we build a simple model to describe the nanoparticles’ distribution in cancerous and normal cells. The finite-difference time-domain (FDTD) method and the Drude-CP (Drude-critical point) model are employed to simulate the nanoparticles in the biological cells. The calculation of total scattering cross-section (TSCS) spectrum and intensity contrast of nanoparticles in cancerous and normal cells, respectively, are compared. The optical immersion technique (OIT) effect of contrast is discussed. The silica/gold nanoshells in cells with different thickness of shells are also compared. Metal nanoparticles are in general optically stable, and resistive to photobleaching. In particular, gold nanoparticles are toxic-free, which makes them very suitable for human body. Therefore, metal nanoparticles have potential for labeling of biological cells and other applications.
Subjects
FDTD method
Drude-CP model
surface plasmon resonance
gold
nanoparticles
nanoshells
cellular labeling
SDGs
Type
thesis
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