Effect of Panax notoginseng Fermented with Lactic Acid Bacteria on the Human Hepatoma Cells in SCID Mice
Date Issued
2006
Date
2006
Author(s)
Su, Chen-Chiang
DOI
zh-TW
Abstract
Panax notoginseng (Burk.) F. H. Chen, is one of the most famous traditional Chinese medicines. It belongs to the Araliaceae family. Its medicinal properties are sweet and slightly bitter in flavor and warm in nature. The main bioactive compound was regarded as saponins, and was used for treatment of diseases of cardiovascular, central nervous, and hematopoietic systems. Cancer prevention and hepatoprotection by saponins have received increasing attention. It has been found that the ginsenosides could be transformed by human intestinal bacteria to metabolites with improvement of anti-carcinogenic activities. Biotransformation of saponins of P. notoginseng by lactic acid bacteria (LAB) is a possible way to enhance its anti-hepatoma activity. The objectives of this study are (1) to establish the optimal conditions, such as fermentation time, temperature and pH value, for the lactic acid fermentation of P. notoginseng, to enhance the anti-hepatoma function of the fermented products; (2) to investigate the bioactive components in the fermentation products which are responsible for the anti-hepatoma activity; (3) to elucidate the inhibitory effect of the fermented products on the growth of hepatoma cells by means of in vitro and in vivo studies.
The present study established the optimal fermentation conditions for the inhibitory effects of fermentative broth on hepatoma cells. The best inhibitory effects were reached after fermentation with LAB for 48 hours at 37℃, and leaving the pH value uncontrolled when carrying out the fermentation in a 6.6 liter bioreactor. The IC50 of Hep 3B and Hep G2 were 226 μg/ml and 149 μg/ml, respectively. Meanwhile, the identification of the saponins in the fermentative broth of P. notoginseng
was elucidated by using LC–ESI-MS/ MS and CID. Based on matching their detection of the saponin molecular weight, and the fragment ions of the molecular ion obtained in the CID experiments with data reported in the literature. Further study will focus on the animal experiments.
Subjects
三七
乳酸菌
皂苷
抗肝腫瘤
SCID mice
Panax notoginseng
LAB
saponins
anti-hepatoma
SDGs
Type
thesis
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