In situ hybridization of Helobdella (leech) embryos
Journal
Cold Spring Harbor Protocols
Journal Volume
4
Journal Issue
4
Date Issued
2009
Author(s)
Abstract
INTRODUCTION The purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus . The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.
SDGs
Type
journal article