Inhibition of Dengue Viral Infection of Human-DC-SIGN-transfected Cells by RANi
Date Issued
2007
Date
2007
Author(s)
Hsu, Ya-Ge
DOI
zh-TW
Abstract
Dengue fever is an important epidemic disease distributing in tropic and sub tropic region. Many countries and WHO work hard to prevent its development. To investigate dengue virus, many researchers have devoted themselves to finding suitable animal model. Until now no animal model is comparable with human case. Dengue virus is infected in human body by interacting with DC-SIGN molecule on dendritic cells. There was an interesting result from previous study at Dr. Wu’s lab. Human DC-SIGN（hDC-SIGN）was stably expressed in the murine B lymphoma cell line M12 which cannot be infected by dengue virus originally. They found those M12 cells carrying hDC-SIGN became susceptible for dengue virus. Both dengue viral RNA and protein level increased within 48 hour post-infection. The number of dengue viral particle in supernatant also arose greatly. This result suggests that the expression hDC-SIGN is sufficient for dengue virus infection, entry and replication in murine cells. To further confirm this point, we design an RNA interference（RNAi）approach to knock down hDC-SIGN expression. By decreasing the expression of hDC-SIGN in M12 cells, dengue virus can not infect those cells anymore. The result may support the importance and the possibility of hDC-SIGN on establishing the murine model for dengue viral study. In this study we find No. 90 RNAi sequence is most efficient to reduce 80% of surface hDC-SIGN expression. Surprisingly, an RNAi sequence which can also knock down 80% of hDC-SIGN by published report previously, doesn’t work well in this experiment. Dengue viral protein could not be detected in hDC-SIGN-overexpressed M12 cells within 48 hours post-infection because of the decreased level of hDC-SIGN by RNAi（No. 90）. These data show that murine B lymphoma cell line M12 could gain the ability of susceptible for dengure virus with hDC-SIGN on its cell surface. On the other hand, level of hDC-SIGN could be down-regulated by RNAi and thus M12 cells cannot be infected by dengue virus. These findings conclude the importance of hDC-SIGN to make non-human cell become suitable for dengue viral infection.
Subjects
DC-SIGN
登革病毒
核糖核酸干擾
Dengue virus
RNA interference
SDGs
Type
other
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