行政院國家科學委員會專題研究計畫成果報告:研發綠色螢光基因轉殖微生物生物偵測器作為偵測有機污染物之研究
Date Issued
2003
2003-07-31
Date
2003
2003-07-31
Author(s)
DOI
912211E002096
Abstract
A biosensor is composed of a biological sensing component, which recognizes chemical or
physical changes, couple to a transducing element that produces a measurable signal in response to
the environmental insult. Genetically engineered cell-based biosensors can elicit a response in the
presence of an environmental insult by coupling the sensing element to a reporter gene fusion,
which upon expression produces a readily measurable signal. The sensing element is often
composed of regulatory proteins and promoter. The specificity of these sensing elements for the
environmental insult confers selectivity to the system, while the reporter protein determines the
systems’s sensitivity and detection limits.
This project studied the construction, laboratory characterization, and environmental testing of
genetically engineered GFP-based bacterial biosensors that detect specific organic pollutants (e.g.,
benzene). The reporter plasmid in which expression of gfp gene for jellyfish Aequorea victoria
green fluorescence protein (GFP) will be placed directly under the control of the specific promoter.
The primary advantage of GFP as a reporter protein is its autofluorescence, and therefore, its use
does not require the addition of cofactors or exogenous substracts to produce fluorescence.
Therefore, such GFP-based biosensors might provide even real time monitoring environmental
pollutants.
We have successfully constructed a prokaryotic GFP-based vector. This vector is used for
developing the benzene biosensor. The results indicated that the GFP-based vector has almost no
fluorescence background and high stability. In addition, we have successfully isolated and amplified
one of the promoter fragments that are essential for establishing the benzene biosensor.
Subjects
biosensors
green fluorescence protein (GFP)
organic pollutants
SDGs
Publisher
臺北市：國立臺灣大學生物環境系統工程學系暨研究所
Type
report
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