伏馬鐮孢毒素B1 (FUMONISIN B1) 對豬的免疫毒害： 肺泡巨噬細胞與週邊血液單核細胞的致害及其作用機制(3/3)
Date Issued
2002
Date
2002
Author(s)
DOI
902313B002297
Abstract
by Fusarium species in which Fumonisin B1
(FB1) is the most common corn, corn product,
and feedstuff contaminant. It has been shown
that it may cause immunological effects in
poultry, mice, and rats. To evaluate the potential
effect of FB1 on swine immune functions, swine
peripheral blood mononuclear cells (SPBMC)
and alveolar macrophages (SAM) were
incubated with FB1 in vitro at various
concentrations for different time periods. The
cell viability and various functions of both cells
were examined. Additionally, the SAM and
SPBMC were activated by LPS and Con A,
respectively, to compare the susceptibility of
resting and activated cells to FB1 toxicity. For
SPBMC, the parameters examined included cell
viability; DNA, RNA, and protein synthesis;
mRNA expression of IL-1b, IL-2, IL-8, IL-10,
IL-12, and IFN-g; and IL-2 production. For
SAM，the parameters examined included cell
viability; phagocytic and microbicidal activities;
O2
- and H2O2 production; mRNA expression of
IL-1b, IL-8, and TNF-a; and TNF-a and PGE2
production. In addition, the effects of FB1 on the
metabolism of sphinganine (Sa) and sphingosine
(So) of SPBMC and SAM were also evaluated.
The results showed that under the selected doses
and incubation periods FB1 could cause
significant reduction in the cell viability; DNA,
RNA, and protein synthesis; and IL-2 production
in either Con A-activated SPBMC or non-Con
A-activated SPBMC. For the mRNA expression
in non-Con A-activated SPBMC, it was reduced
at the early stage but then increased in IL-1b,
IL-2, IL-8, IL-10, and IFN-g, but it was
increased before 36 hours of incubation (HOI)
and reduced thereafter in IL-12 and TNF-a. The
cytokine mRNA expression in Con A-activated
SPBMC was inconsistent, among which IL-1b
was constantly increased in most of the time
points; significant variations were present in
IL-2, IL-8, IL-12, and IFN-g; and it was
decreased in TNF-a. For the medium control,
the level of cytokine mRNA expression in Con
A-activated SPBMC was lower than that in
non-Con A-activated SPBMC. For the
sphingolipid metabolism, the content of Sa and
So and the Sa/So ratio were elevated following
24 HOI in both 20 and 50 mg/ml FB1-treated
SPBMC. As for the SAM, the cell viability was
increased at the early stage but reduced later in
both LPS-activated and non-LPS-activated
groups with the former occurring earlier. The
potential of O2
- and H2O2 production were all
reduced in both groups with O2
- more prominent.
The ability of SAM to engulf Candida albicans
was reduced in both LPS and
Fumonisins are secondary metabolites produced
non-LPS-activated FB1-treated groups in a
time-dependent manner with the former more
evident. The ability of SAM to kill the engulfed
C. albicans was also reduced in both groups in a
dose-dependent manner with the LPS-activated
FB1-treated group more apparent. As for the
IL-1b mRNA expression, it was reduced initially
followed by increase in both groups; for IL-8, it
was slightly increased initially and then became
plateau in the LPS-activated FB1-treated group,
but it was slightly decreased initially and then
became increased in the non-LPS-activated
FB1-treated group; for TNF-a, no consistent
results were revealed in both groups. FB1
treatment caused the reduction in the level of
TNF-a production in the LPS-activated SAM but
caused initial reduction followed by elevation in
the non-LPS-activated SAM. FB1 treatment
induced the increase in the level of PGE2
production in the LPS-activated SAM but caused
initial increase followed by decrease in the
non-LPS-activated SAM. For the sphingolipid
metabolism, the content of Sa and So and the
Sa/So ratio were elevated in a time-dependent
manner in the LPS-activated FB1-treated SAM.
The results indicate that FB1 could indeed cause
cell death, multiple functional alterations, and
disturbance in the sphingolipid metabolism of
SPBMC and SAM. It is anticipated that
long-term consumption of FB1-contaminated
feed has the potential to interfere with the
immune regulation in pigs with subsequent
reduction in disease resistance. The immune
deregulation is possibly related to the changes in
sphingolipid metabolism.
Subjects
Fumonisin B1
Immunotoxicity
Sphingolipid
Peripheral blood mononuclear cells
Alveolar macrophages
Swine
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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