A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response
Resource
PLANTA, 231(6), 1459-1473
Journal
Planta
Pages
1459-1473
Date Issued
2010
Date
2010
Author(s)
Abstract
Abiotic stresses such as cold, water deficit, and salt stresses severely reduce crop productivity. Tomato (Solanum lycopersicum) is an important economic crop; however, not much is known about its stress responses. To gain insight into stress-responsive gene regulation in tomato plants, we identified transcription factors from a tomato cDNA microarray. An ABA-responsive element binding protein (AREB) was identified and named SlAREB. In tomato protoplasts, SlAREB transiently transactivated luciferase reporter gene expression driven by AtRD29A (responsive to dehydration) and SlLAP (leucine aminopeptidase) promoters with exogenous ABA application, which was suppressed by the kinase inhibitor staurosporine, indicating that an ABA-dependent post-translational modification is required for the transactivation ability of SlAREB protein. Electrophoretic mobility shift assays showed that the recombinant DNA-binding domain of SlAREB protein is able to bind AtRD29A and SlLAP promoter regions. Constitutively expressed SlAREB increased tolerance to water deficit and high salinity stresses in both Arabidopsis and tomato plants, which maintained PSII and membrane integrities as well as water content in plant bodies. Overproduction of SlAREB in Arabidopsis thaliana and tomato plants regulated stress-related genes AtRD29A, AtCOR47, and SlCI7-like dehydrin under ABA and abiotic stress treatments. Taken together, these results show that SlAREB functions to regulate some stress-responsive genes and that its overproduction improves plant tolerance to water deficit and salt stress. © Springer-Verlag 2010.
Subjects
ABA-responsive element binding protein; Abscisic acid; Salinity; Water deficit
Other Subjects
abscisic acid; Arabidopsis protein; basic leucine zipper transcription factor; dehydrin proteins, plant; luciferase; plant DNA; RD29a protein, Arabidopsis; sodium chloride; vegetable protein; water; adaptation; amino acid sequence; Arabidopsis; article; chemistry; DNA responsive element; drug effect; gene expression regulation; genetics; metabolism; molecular genetics; physiological stress; physiology; protein binding; protein tertiary structure; protoplast; reporter gene; salinity; signal transduction; tomato; transcription initiation; transgene; Abscisic Acid; Adaptation, Physiological; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Basic-Leucine Zipper Transcription Factors; DNA, Plant; Gene Expression Regulation, Plant; Genes, Reporter; Luciferases; Lycopersicon esculentum; Molecular Sequence Data; Plant Proteins; Protein Binding; Protein Structure, Tertiary; Protoplasts; Response Elements; Salinity; Signal Transduction; Sodium Chloride; Stress, Physiological; Transcriptional Activation; Transgenes; Water; Arabidopsis; Arabidopsis thaliana; Lycopersicon esculentum; Solanum
Type
journal article
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