Auto-searching Erythrocytes Harmonic Generation Microscopy Method
Date Issued
2013
Date
2013
Author(s)
Hsieh, Chia-Hung
Abstract
Blood routine examination is important to check the health screening program, through the image of blood smear microscopy about the state of blood, such as red blood cells (RBCs), the white blood cells (WBCs), platelet count, hematocrit (Hct) are common examination in laboratory medicines, it requires a draw of blood, which is an invasive method to patients. It cost patients’ time to wait fo results and may be the subject of environmental factors on in vitro led todeterioration in turn affect the accuracy check.
With the advancement of technology, non-invasive medical imaging inspection technology innovation, because of the nonlinear optical microscopy has sub-micron level tomography three-dimensional image resolution , has been widely used in biological observation and research. In the current nonlinear optical microscopy, harmonics-based in vivo optical virtual biopsy has three-dimensional cellular-level image, the system will not cause harm to the organism, but also without the use of stain .Therefore, the research team use this system for real-time observation and analysis of in vivo human blood cells, shape, flow and shape. We use arm as observation point, laser focus to subcutaneous capillaries. Before experiment, the participant loads his arm on the translation stage, operator adjusts the altitude of object and translation stage by manual, With three dimensional searching , program controls the laser focus to further observation of subcutaneous blood capillaries. As the capillaries from the epidermis only 200μm, operator adjustments coarse or fine adjustment knob of objective and controls translation stage powerfully , the capillary could be ignored, in addition, due to the capillary was three-dimensional distribution, the situation is prone to repeat the search resulted in poor efficiency, therefore, this thesis published “Algorithm of auto-searching subcutaneous capillaries”, not only can improve search efficiency, but also reduce the burden on the operator can be avoided to contact with patients.
Subjects
倍頻顯微術
自動搜尋
演算法
SDGs
Type
thesis
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