The study of anti-apoptotic genes iap2 and iap3 of Lymantria xylina multiple nucleopolyhedrovirus, (LyxyMNPV): cloning, sequencing, expression and comparison
Date Issued
2006
Date
2006
Author(s)
Nai, Yu-Shin
DOI
zh-TW
Abstract
The anti-apoptosis genes of baculoviruses are essential genes for viral propagation in their susceptible host cells. There are two main groups of antiapoptotic genes in baculovirus genome, p35 and iap gene family (iap1~iap5), the latter is more wildly distributed in baculovirus. According to the results of PCR amplification and Southern blot hybridization, Lymantria xylina multiple nucleopolyhedrovirus (LyxyMNPV) contains only two iap genes ly-iap2 (687 bp) and ly-iap3 (450 bp). The sequences of iap2 and iap3 of LyxyMNPV had high identities with those of L. dispar MNPV (LdMNPV), about 81% and 86%, respectively. The results of Northern blot hybridization revealed that, a 1.95-kb transcript of ly-iap2 was first detected at 72 h postinfection (pi) and 1.04- and 2.09-kb transcripts of ly-iap3 were first detected at 48 h pi. The iap genes of Perina nuda MNPV (PenuMNPV) had been also cloned and sequenced. LyxyMNPV and PenuMNPV were compared with other known baculovirus iap genes for phylogenetic analysis. The trees showed that LyxyMNPV was closely related to LdMNPV, in addition, LyxyMNPV, PenuMNPV and Autographa californica MNPV (AcMNPV) were located seperately in three different clusters. LY-IAP3 polyclonal antibody had been prepared and used to detect the expression of LY-IAP3 in LyxyMNPV-infected IPLB-LD 652Y-7 cells (LyxyMNPV permissive cells) and NTU-SL7B cells (LyxyMNPV non-permissive cells) by indirdct fluorescence immunoassay (IFIA). LY-IAP3 could be expressed in the cytoplasm of both cells; therefore, the role of LY-IAP3 needs to be further elucidated.
Subjects
黑角舞蛾核多角體病毒
抑制細胞凋亡基因
LyxyMNPV
iap
Type
thesis
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