The effect of interaction between metal ion and microalgae toward the performance of algal cell disruption by ultrasonication
Date Issued
2015
Date
2015
Author(s)
Wei-Wang
Abstract
Ultrasonication is a cell disruption process at low energy effeciency. The goal of research here is to find the effect of interaction between metal ion and microalgae toward the performance of cell disruption by ultrasonication .Three factors were assumed to affect the performance here : relative proximity of the bubble to the cell based on electrostatic effect, size of aggregates and structure of aggregates. To check which factor above is dominant one, we dosed K+,Ca2+,Al3+ to chlorella vulgaris cultured in Bold’s Basal Medium at 25oC, followed by measuring degree of disruption after ultrasonication. The one without dosing is used as control.. Three indicators : increase of Chla & COD after sonication, degree of cell disruption by SYTOX fluorescence staining test were used to quantify the degree of disruption. In the result the degree of cell disruption follow : K+ > Control > Ca2+ >Al3+ sample. We can deduce that surface charge of cell and microbubble have small effect on the disruption efficiency, however; cell aggregate with large size and compact interior have low efficiency. We hypothesize that microbubble could not be effective generated inside compact aggregate, which lead to low cell disruption efficiency.
Subjects
microbubble
metal ion
aggregate
cell disruption
ultrasonication
Type
thesis
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