Monoclonal antibody for the detection and identification of a phytoplasma associated with rice yellow dwarf
Journal
European Journal of Plant Pathology
Journal Volume
101
Journal Issue
5
Pages
511-518
Date Issued
1995
Author(s)
Chang, F.-L.
Chen, C.-C.
CHAN-PIN LIN
Abstract
One stable hybridoma clone, 247B11, secreting specific monoclonal antibody (MA) against the mycoplasmalike organism (MLO), newly be termed phytoplasma, associated with rice yellow dwarf (RYD) was produced by employing an immunization scheme for inducing the immunological tolerance of mice to rice antigens prior to the administration of RYD-phytoplasma immunogens. Neonatal BALB/c mice were first injected with nontarget rice antigens present in the immunogen preparation and were immunized intrasplenically with RYD-phytoplasmaenriched antigens prepared by Percoll density-gradient fraction 6 wk later. The MA was of the IgG1 class. With this MA, RYD-phytoplasma in diseased rice was specifically detected by indirect enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining and tissue-blotting techniques. Antibody titer determined by indirect ELISA for hybridoma-culture supernatant was 5120. The antibody recognized two polypeptides, 16 kDa and 41 kDa of RYD-phytoplasma determined by western blotting. RYD-phytoplasma was differentiated serologically from the phytoplasmas associated with sweetpotato, peanut, loofah, paulownia, and Ipomoea obscura witches' broom, aster yellows (NJ strain), elm yellows, and sugarcane white leaf both in indirect ELISA and immunofluorescent staining. © 1995 Kluwer Academic Publishers.
SDGs
Type
journal article