Mixed-Monosaccharides Encapped Gold Nanoparticles to Detect and Purify Corresponding Binding Proteins
Date Issued
2006
Date
2006
Author(s)
Chuang, Shiuan-Ting
DOI
zh-TW
Abstract
Recent research results show that carbohydrates play many biological roles such as information carriers. Oligosaccharides on the glycoproteins or glycolipids display highly specific recognition because of their extremely complicated and various stereoisomers. For example, oligosaccharides Lewis antigens have been shown in mammalian systems to be important recognition determinants in cell–cell interactions. Therefore, many scientists devote their efforts to carbohydrate research. However, the advancement of the field is highly hampered by rather difficult and challenging synthesis of oligosaccharides. To circumvent the synthetic dilemma, we plan to identify the key monosaccharides of oligosaccharides, which are pivotal to the biological functions, and immobilize them on 32-nm gold nanoparticles (GNPs) as multivalent systems to achieve the desired effects. Tetragonolobus purpureas (LTL) and L-selectin, Lewis binding lectins, was chosen to demonstrate our molecular. Changes of surface plasma resonance (SPR) absorption were carefully monitored and used as indication of binding.
Previous studies showed that the binding strength of
Subjects
蛋白質純化
金奈米粒子
purify protein
gold nanoparticles
Type
thesis
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