Protective effect of Acacia confusa bark extract and its active compound gallic acid against carbon tetrachloride-induced chronic liver injury in rats
Journal
Food and Chemical Toxicology
Journal Volume
47
Journal Issue
6
Pages
1385-1392
Date Issued
2009
Author(s)
Abstract
Acacia confusa Merr. (Leguminosae), a species native to Taiwan, is widely distributed on the hills and lowlands of Taiwan, and has been traditionally used as a medicine. The hepatoprotective effects of A. confusa bark extract (ACBE) and its active constituent gallic acid were evaluated against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. CCl4-induced hepatic pathological damage and significantly increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) in plasma, and cytochrome P4502E1 (CYP2E1) protein expression in hepatic samples, and decreased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) in erythrocytes. Treatment with ACBE, gallic acid or silymarin could decrease significantly the AST, ALT, and MDA levels in plasma, and CYP2E1 expression in liver tissues, and increase the activities of SOD and GPX in erythrocyte when compared with CCl4-treated group. Liver histopathology also showed that ACBE, gallic acid or silymarin could significantly reduce the incidence of liver lesions induced by CCl4. These results suggested that the ACBE and gallic acid exhibit potent hepatoprotection against CCl4-induced liver damages in rats, and the hepatoprotective effects of ACBE and gallic acid may be due to the modulation of antioxidant enzymes activities and inhibition of lipid peroxidation and CYP2E1 activation. Crown Copyright ? 2009.
Subjects
Acacia confusa; Carbon tetrachloride; CYP2E1; Gallic acid; Hepatoprotective effect
SDGs
Other Subjects
acacia confusa extract; alanine aminotransferase; aspartate aminotransferase; carbon tetrachloride; catalase; cytochrome P450 2E1; gallic acid; glutathione peroxidase; lipid; malonaldehyde; plant extract; silymarin; superoxide dismutase; unclassified drug; Acacia; acacia confusa; alanine aminotransferase blood level; animal cell; animal experiment; animal model; animal tissue; article; aspartate aminotransferase blood level; bark; comparative study; controlled study; drug megadose; enzyme activation; enzyme activity; erythrocyte; histopathology; lipid peroxidation; liver; liver histology; liver injury; liver protection; low drug dose; male; nonhuman; plasma; protein expression; rat; Acacia; Animals; Antioxidants; Blotting, Western; Carbon Tetrachloride Poisoning; Cytochrome P-450 CYP2E1; Erythrocytes; Gallic Acid; Hepatitis, Toxic; Lipid Peroxidation; Liver; Liver Function Tests; Male; Plant Bark; Plant Extracts; Rats; Rats, Sprague-Dawley; Thiobarbituric Acid Reactive Substances; Acacia confusa; Fabaceae; Rattus
Type
journal article