Studying the Galvanotaxis Behavior of Cells and the Underlying Mechanism
Date Issued
2002
Date
2002
Author(s)
DOI
902214E002001
Abstract
The characteristic of a cell that it moves directionally in an applied electric field (EF)
is termed “galvanotaxis ”. Though the
characteristic is generally taken as a common
behavior, the underlying mechanism is not
yet clarified. This study investigated the
galvanotaxis of normal human fibroblast
(NHDF) in an EF and tried to reveal the
underlying mechanism through seeding them
on different substrata or treating them with
several inhibitors separately. NHDF moved
toward anode (the average directedness
was –0.37) in 1 V/cm EF with a speed of 4
m/h on glass slips (EF controls). NHDF
remained their directional migration toward
anode on other four substrata (TCPS,
collagen, gelatin and chitosan) with speed
over 60 m/h. NHDF had the largest average
directedness of –0.51 on TCPS. Inhibition
the formation of F-actin, the classical protein
kinase C (cPKCs) and reactive oxygen
species (ROS) abolished the directional
migration of NHDF in EF on glass slips with
average directedness lower than –0.07. But
chelating the extracellular calcium ions made
NHDF moved more devotedly toward anode
with an average directedness of –0.70. The
chemical-treated NHDF moved slower than
the EF controls with speeds less than
20m/h.
Subjects
fibroblast
galvanotaxis
substratum
signal transduction
Publisher
臺北市：國立臺灣大學化學工程學系暨研究所
Type
report
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